Phytochemical Content Analysis of Simplex and Ethanol Extract of Pandanus amaryllifolius Roxb. Leaves as Potential Natural Ingredients
DOI:
https://doi.org/10.52120/jlik.v4i1.106Kata Kunci:
Pandanus amaryllifolius Roxb, fitokimia, refluks, etanol 96%, metabolit sekunder, flavonoid, alkaloid, terpenoidAbstrak
This study aimed to compare the secondary metabolite profiles of crude drug (simplicia) and refluxed ethanol extract of Pandanus amaryllifolius Roxb. leaves through qualitative phytochemical screening. Fresh fragrant pandan leaves were processed into dried simplicia, powdered, and extracted using the reflux method with 96% ethanol (1:10 w/v) at ±78°C for 3 hours. The extract was concentrated using a rotary evaporator and analyzed for alkaloids, flavonoids, phenolics, saponins, terpenoids, and steroids based on standard color reactions and precipitate formation. The drying process resulted in a weight loss of 37.58%, indicating effective moisture removal, while extraction yielded 9% thick extract. Phytochemical screening revealed the presence of alkaloids, flavonoids, and terpenoids in both simplicia and extract, whereas phenolics, saponins, and steroids were not detected under the applied conditions. These findings indicate that 96% ethanol reflux extraction effectively isolates polar and semi-polar bioactive compounds without significant thermal degradation. The results provide a scientific basis for the development of pandan-based phytopharmaceuticals and natural cosmetic formulations.
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Adhamatika, A., Murtini, E. S., & Sunarharum, W. B. (2021). The effect of leaf age and drying method on physico-chemical characteristics of pandan (Pandanus amaryllifolius Roxb.) leaves powder. IOP Conference Series: Earth and Environmental Science, 733(1). https://doi.org/10.1088/1755-1315/733/1/012073
Azhar, A. N. H., Amran, N. A., Yusup, S., & Mohd Yusoff, M. H. (2022). Ultrasonic Extraction of 2-Acetyl-1-Pyrroline (2AP) from Pandanus amaryllifolius Roxb. Using Ethanol as Solvent. Molecules, 27(15), 1–18. https://doi.org/10.3390/molecules27154906
Bhuyan, B., & Sonowal, R. (2021). An overview of Pandanus amaryllifolius Roxb.exLindl. and its potential impact on health. Current Trends in Pharmaceutical Research, 8(1), 1–20. https://dibru.ac.in/wp-content/uploads/2021/09/08-CTPR-Review-BB-06.pdf?2023120908
Buddhakala, N., & Yongkhamcha, B. (2025). Phytochemical constituents, and antioxidant, antidiabetic and anti-inflammatory activities of the extracts from Pandanus amaryllifolius Roxb. Journal of Herbal Medicine, 54(September), 101062. https://doi.org/10.1016/j.hermed.2025.101062
Burhana, N. A., Akbar, M. F., & Putri, A. S. (2023). Comparison of Pandan Leaf Extract ( Pandanus Amaryllifolius ) Using Ethanol and N-Hexane to The Content of Bioactive Compounds The 3 rd International Conference on Sustainable Health Promotion ( ICOSHPRO ) Comparison of Pandan Leaf Extract ( Pandanus Amar. August, 30–31.
Ghazali, A. M., Umar, N. B., & Hussain, N. (2024). Application of Different Aqueous Ultrasound-assisted Extraction Process Parameters on the Quality of Pandan (Pandanus amaryllifolius Roxb) Leaves Extract. Journal of Biochemistry, Microbiology and Biotechnology, 12(SP1), 9–11. https://doi.org/10.54987/jobimb.v12isp1.924
Harborne, J. B. (1987). Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Bandung: ITB Press.
Harun Nurashid, H. N. H., Samsu Baharudin, A., Mohd Amin, N. A., Mad Nordin, M. F., & Hafid, H. S. (2025). Progress on the studies of Pandanus amaryllifolius, the cultivation in Malaysia and its application in various fields. Advances in Agricultural and Food Research Journal, 6(2). https://doi.org/10.36877/aafrj.a0000590
Kementerian Kesehatan Republik Indonesia. (2020). Farmakope Herbal Indonesia (Edisi II). Jakarta: Direktorat Jenderal Kefarmasian dan Alat Kesehatan, Kementerian Kesehatan Republik Indonesia.
Kementerian Kesehatan Republik Indonesia. (2020). Farmakope Indonesia (Edisi VI). Jakarta: Direktorat Jenderal Kefarmasian dan Alat Kesehatan, Kementerian Kesehatan Republik Indonesia.
Mohamed Zakaria, M., Zaidan, U. H., Shamsi, S., & Abd Gani, S. S. (2020). Chemical Composition of Essential Oils From Leaf Extract. 24(1), 87–96.
Nurbaiti, N., Fitri, Y., Fitriani, F., Humaira, W., & Triwibowo, C. (2024). Phytochemical Composition and Antioxidant Properties of Avocado (Persea americana) Seed Extract from Aceh, Indonesia: Implications for Antihyperlipidemic Use in Postmenopausal Women. Malacca Pharmaceutics, 3(1), 1–9
Qomarudin, Q. (2023). Extraction of Pandanus amaryllifiolus Roxb.: A review of methods and the influencing factors. Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering, 6(1), 72–87. https://doi.org/10.21776/ub.afssaae.2023.006.01.7
Qomarudin, Q., Murtini, E. S., Yunianta, Y., & Witono, Y. (2025). Optimization Microwave-assisted Extraction (MAE) for Extracting Antioxidant Compounds and 2acetyl-1pyrroline (2AP) from Pandan (Pandanus amarillyfolius Roxb) Leaf. BIO Web of Conferences, 165. https://doi.org/10.1051/bioconf/202516505006
Rohaeni, E., Ulfah, T., Duhita, R., Utama, D., & Ramadiyanti, M. (2025). Trehalose : A Key Stabilizer for Pandan Leaf ( Pandanus amaryllifolius Roxb ) Extract Powder.
Sa’adah, S. M., Putri, F. R., Ibtisam, A. A., Arrohmah, R. S., & Fitriyah, F. (2023). Phytochemical analysis of secondary metabolite compounds of Pandanwangi leaf extract (Pandanus amaryllifolius). Journal of Natural Sciences and Mathematics Research, 9(2), 135–142. https://doi.org/10.21580/jnsmr.2023.9.2.16421
Thanebal, S. A. P., Vun-Sang, S., & Iqbal, M. (2021). Hepatoprotective effects of Pandanus amaryllifolius against carbon tetrachloride (CCl4) induced toxicity: A biochemical and histopathological study. Arabian Journal of Chemistry, 14(10), 103390. https://doi.org/10.1016/j.arabjc.2021.103390
Wahyuni, D. K., Nuha, G. A., Atere, T. G., Kharisma, V. D., Tari, V. S., Rahmawati, C. T., Murtadlo, A. A. A., Syukriya, A. J., Wacharasindu, S., Prasongsuk, S., & Purnobasuki, H. (2024). Antimicrobial potentials of Pandanus amaryllifolius Roxb.: Phytochemical profiling, antioxidant, and molecular docking studies. PLoS ONE, 19(8), 1–20. https://doi.org/10.1371/journal.pone.0305348
Wakte, K. V., et al. (2009).
Occurrence of 2-acetyl-1-pyrroline in Pandanus amaryllifolius Roxb.
Journal of Agricultural and Food Chemistry, 57(9), 3837–3842.
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Hak Cipta (c) 2026 Ayu Nurhalimah, Nikadek Alya Saraswati Dewi, Tara Nia Shalma Nugraha, Anjelika Mesa Putri, Desiva Muspita Ayu, Resi Nopribulin, Iin Vita Noprianti (Author)

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